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The flow rate for each run started at 0.2 ml/min for 5 min, followed by a gradient starting at 0% B and increasing linearly to 100% B over the course of 45 min with a flow rate of 0.7 ml/min, followed by an isocratic gradient of 100% B for 17 min at 0.7 ml/min before equilibrating for 8 min at 0% B with a flow rate of 0.7 ml/min

it's an iterative process that demands careful observation and data analysis

Louis helps to address this question

iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundao para a Cincia e Tecnologia/Ministrio da Educao e Cincia, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is acknowledged

It has a longer duration of action in the body compared to other forms and is often chosen when a sustained release of B12 is desired